DNA VIRUSES DNA (genome) replication strategies similar in all in addition to similar to ho

DNA VIRUSES DNA (genome) replication strategies similar in all in addition to similar to ho www.phwiki.com

DNA VIRUSES DNA (genome) replication strategies similar in all in addition to similar to ho

Lesley, Jeremy, Morning Show Host has reference to this Academic Journal, PHwiki organized this Journal DNA VIRUSES DNA (genome) replication strategies similar in all in addition to similar to host ssDNA becomes dsDNA 5’ to 3’ synthesis; need as long as primer Variety of enzymes of host or viral origin : DNA polymerase (proofreading), helicases, ss binding proteins, ligases In nucleus except as long as poxviruses Phage T4 replisome Replication Challenges as long as DNAViruses Access to nucleus Competing as long as nucleotides Cell cycle control in eucaryotes – S phase dependent materials as long as some Primer removal in addition to replacement (completing ends)

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Transcriptional/translational challenges Access to RNA polymerase Monogenic expression in eukaryotes Temporal control of gene expression Competition with host as long as ribosomes Bacteriophages: T4 Linear dsDNA – ~ 1.2 x 10^8 d (>280 genes) circular permuted terminally redundant http://www.brunel.ac.uk/depts/bl/blst/emma/molecgen/virus/lytic/lytfrm.htm

http://www.brunel.ac.uk/depts/bl/blst/emma/molecgen/virus/lytic/lytfrm.htm Concatemer as long as mation in addition to packaging of headful genome What affect does T4 infection have on macromolecular synthesis in the cell What MOI would you use How would you measure DNA synthesis RNA synthesis Protein synthesis How can you distinguish between phage in addition to host DNA synthesis How can you distinguish between phage in addition to host RNA synthesis

RNA protein DNA Rel conc time 0 RNA production in cell Temporal control of transcription Immediate early: will occur in presence of ps inhibitor What RNA-P is used Delayed early – needs protein synthesis in addition to be as long as e DNA replication Late – after DNA replication begins – structural proteins T4 changes host RNA-P RNA-P – 4 subunits plus sigma factor IE uses host enzyme but at promotors that differ from E. coli (high affinity) IE gene products modifies (ADPr) RNA-P to recognize DE promotors Antitermination Nucleases (host DNA in addition to tRNA) Membrane repair

DE further changes to RNA-P Antisigma factor (ASiA) Activator proteins Phage tRNAs Nucleotide metabolism DNA replication Late requires different sigma factors T4 genome – also 127 ORFs of unknown fucntion

T7 control Linear dsDNA ~ 25 x 10^6d Unique with TR – how is this as long as med Genes are in order of entry on chromosome T7 promotors differ IE – host polymerase Creation of a new polymerase/inactivation of host polymerase T7 polymerase promoter often used in gene cloning as long as control of expression Papovaviruses Papilloma/Polyoma/Vacuolating agent Bidirectional replication from single ori (similar to Bacteria) Early to late strategies T ags in SV40 enhance first in addition to then suppresses early; E ag in BPV is an enhancer as long as late genes Mutations in T or Eag/transition lead to tumors

How do DNA Viruses Get cells out of G1 in addition to into S phase Inactivate Rb/p53 – cell cycle regulators SV40 uses T ag against p53 p53 inactivation probably stops apoptosis Multiple functions as long as T ag increases genome potential HPV Transcription using host RNA-P Multiple promotors some with overlapping reading frames Alternative splicing – more genes as long as your genome Adenovirus – 5’protein primer Linear dsDNA 20-30 x 10^6 d Terminal protein linked to 5’nucleotide Sequential replication from linear DNA No Okazaki fragments This is now a template

Inverted terminal repeats Adenovirus – transcription Monogenic proteins with individual promotors Uses host RNA-P Multliple splicing of mRNA yields different proteins E1A is IE gene- activates at other E promotors

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Poxvirus: DNA with a complex morphology Large genomes – 130 n- 240 x 10^6d Denatured genome is ss circle Replicates in cytoplasm Brings in RNA-P; mRNA is capped Makes all replicating enzymes DNA replication Herpes Simplex Virus Tegument – ~ 18 proteins Access to nucleus TIF (VP16 /UL48 ) trans inducing factor binds with host factors to begin transcription 500 – 1000 copies/virion Determines tissue tropism VHS (UL41) degrades preexisting mRNA but is stopped so virus can work

Families of Herpes viruses Temporal expression of genes Alpha in addition to Beta proteins Alpha ICP27 – blocks host RNA splicing Immune escape (MHC1 downregulation) Turn on Beta genes Beta DNA replication (polymerae,binding proteins, helicase/primase) Thymidine kinase DNA repair proteins Turn on Gamma/off Alpha Gamma Structural proteins Tegument proteins

Protection from host are early products Prevention of apoptosis Use mutants in addition to see affects Cisplatin is apo inducer (+ control) apoptosis wt cisplatin ICP- What do the results show Infect cell with virus in presence of Actinomycin D (inhibits transcription from DNA) Add S-35 methionine in addition to measure ps after 1 hour KSHV v-cyclin/v-FLIP gene gives a single transcript Both cell homologs Cyclin regulates cell cycle FLIP delays apoptosis How are two proteins produced from one message

Lesley, Jeremy Morning Show Host

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