How much DNA amplification has occurred How do scientists analyse these PCR products Agarose Gel Electrophoresis Separates DNA fragments

How much DNA amplification has occurred How do scientists analyse these PCR products Agarose Gel Electrophoresis Separates DNA fragments www.phwiki.com

How much DNA amplification has occurred How do scientists analyse these PCR products Agarose Gel Electrophoresis Separates DNA fragments

Chatterjee, Pallab, Contributing Editor has reference to this Academic Journal, PHwiki organized this Journal 1,000,0001,000,000,000PCR cycles204030TheorecticalyieldActual yield“Plateau effect”No. of DNA copiesHow much DNA amplification has occurredHow do scientists analyse these PCR productsAgarose Gel ElectrophoresisSeparates DNA fragments according to their size

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Step 1 – Agarose is added to buffer in addition to heated to 95ºC (to melt it). After cooling, it is poured into a pre-preparedcasting trayStep 2 – a comb is inserted immediately to create wells as long as loading the samplesStep 3 – the gel is left to setStep 4 – the comb is removed slowly(see set of wells that have been created)Making Agarose GelMaking Agarose GelA fluorophore (DNA SafeView) is added to the gel. This intercalates with the DNA & fluoresces when excited by UV lightLoading DNA samples

Loading DNA samplesSeparating the DNA FragmentsAfter the samples are loaded into the wells, an electric current is applied across the gel in addition to the gel is “run”Separating the DNA Fragments

Separating the DNA FragmentsSeparating the DNA FragmentsVisualising DNAThe UV light excites the DNA SafeView (fluorophore) that is bound to the DNATransilluminator

Chatterjee, Pallab EDN Contributing Editor www.phwiki.com

Chatterjee, Pallab Contributing Editor

Chatterjee, Pallab is from United States and they belong to EDN and they are from  Livermore, United States got related to this Particular Journal. and Chatterjee, Pallab deal with the subjects like Design Engineering; Electrical Engineering; Electronic Components and Semiconductors

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This Particular Journal got reviewed and rated by Oregon State University and short form of this particular Institution is OR and gave this Journal an Excellent Rating.