Image J Protocol For Western blot analysis 2/10/2006 Terry D. Johnson 1. Open Im
Lopez, Rosella, General Manager has reference to this Academic Journal, PHwiki organized this Journal Image J Protocol For Western blot analysis 2/10/2006 Terry D. Johnson 1. Open Image J in addition to , in it, open the .TIF file to be analyzed – in this case, example.tif. 1b. Optional If the image is not already an 8-bit .TIF, go to Image -> Type in addition to select 8-bit. If the image is not dark enough to analyze properly, go to Image -> Adjust -> Brightness/Contrast in addition to adjust the slider bars until the b in addition to s on the image are visible. The pixel values will not be changed, just the view.
This Particular University is Related to this Particular Journal
2. In the main menu, select Analyze > Calibrate. 3. Select the Uncalibrated OD option from the Function: dropdown box, then click OK. 4. A logarithmic plot should appear; move it to the side, but do not close the window.
5. Go back to your .TIF image in addition to select the Rectangle Tool from your toolbox. Box all of the b in addition to s of interest across all of the lanes in addition to hit Crtl-H to bring up a histogram. 6. Carefully outline the first lane with the same tool. Go to Analyze > Gels > Select First Lane. 7. Drag the rectangle that you used as long as the first lane over to the next, in addition to mark it with Analyze > Gels > Mark Next Lane. Dont draw a new rectangle! You want every rectangle to have the same area. Repeat until all the lanes have been marked.
8. Click on Analyze > Gels > Plot Lanes. Youll end up with an image file with plots as long as each of the lanes that you marked. The plot on top is the first lane that you marked. Use Image > Rotate 90 Degrees Right (or Left) in addition to /or Image > Zoom > Out so you can see in addition to work with all of the plots. (OPTIONAL) If you have more than one b in addition to per lane, youll end up with a plot as long as a single lane that has multiple peaks (one as long as each b in addition to ). Youll need to separate them into individual peaks with the Line Tool as demonstrated below on another image. 9. Select the Magic W in addition to tool in addition to select each plot beginning with the first. A box will appear with the Area measurement as long as each plot as it is selected. The area of that histogram plot represents the darkness of each b in addition to in the gel.
10. Once you have selected each b in addition to , you can analyze the area measurements in your spreadsheet of choice.
Lopez, Rosella General Manager
Lopez, Rosella is from United States and they belong to KCEC-FM and they are from Yuma, United States got related to this Particular Journal. and Lopez, Rosella deal with the subjects like Hispanic Interest; Latin Music; Music
Journal Ratings by Baker University
This Particular Journal got reviewed and rated by Baker University and short form of this particular Institution is US and gave this Journal an Excellent Rating.