Lecture 37 Gene regulation: Control of gene expression. (Prokaryotic cells) Gene

Lecture 37 Gene regulation: Control of gene expression. (Prokaryotic cells) Gene www.phwiki.com

Lecture 37 Gene regulation: Control of gene expression. (Prokaryotic cells) Gene

Holifield, Lisa, News Director has reference to this Academic Journal, PHwiki organized this Journal Lecture 37 Gene regulation: Control of gene expression. (Prokaryotic cells) Gene regulation in prokaryotes: 1. By positive control a. with induction of gene expression b. with repression of gene expression 2. By negative control a. with induction of gene expression b. with repression of gene expression. P O A B C R’ active regulator inactive regulator Structural genes E1 E2 E3 Function DNA RNA protein (Prokaryotic) Operon.

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P O Z Y A R’ inactive repressor active repressor Function Tryptophan operon: Tryptophan P O Z Y A R’ Structural genes inactive repressor Without tryptophan: With tryptophan: polymerase P O Z Y A R’ active repressor inactive repressor Function Lactose operon: Lactose P O Z Y A R’ Structural genes active repressor Without lactose: With lactose: polymerase

Glucose low, cAMP high promotor activated Glucose high, cAMP low, promotor not activated Glucose: low Lactose: not present Glucose: low Lactose: present

Fig. 14-6, p. 312 Lecture 38 Gene regulation: Eukaryotic cells, Transcription factors.

Zinc finger.

(Helix-turn-helix)

Regulation of gene expression multicellular organisms: Eukaryotic Gene structure:

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Regulation of gene transcription: activation of a TF by phosphorylation.

Regulation of gene transcription: activation of a TF by removal of inhibitor. Eu-chromatin in addition to Hetero-chromatin:

Fig. 10-2a, p. 213 DNA wound around a cluster of histone molecules Histone tails Linker DNA Nucleosome (10 nm diameter) The End.

Holifield, Lisa News Director

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