Type III Secretion System (TTSS) Pseudomonas aeruginosa How does ExoU function Using genome sequences to get the answer Testing the phospholipase idea: Syringe Loading

Type III Secretion System (TTSS) Pseudomonas aeruginosa How does ExoU function Using genome sequences to get the answer Testing the phospholipase idea: Syringe Loading www.phwiki.com

Type III Secretion System (TTSS) Pseudomonas aeruginosa How does ExoU function Using genome sequences to get the answer Testing the phospholipase idea: Syringe Loading

Hass, Gretchen, Copy Editor has reference to this Academic Journal, PHwiki organized this Journal Molecular interactions between hosts in addition to pathogens Partho Ghosh Department of Chemistry & Biochemistry in addition to Section of Molecular Biology University of Cali as long as nia, San Diego http://pghosh.ucsd.edu Pathogens Host cells Infectious Diseases at the Molecular Level Type III Secretion Needles Kubori et al., Science 280:602 (98) Pathogenic bacterium Human cell

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Type III Secretion System (TTSS) Gram-negative Pathogens of mammals Escherichia coli Pseudomonas aeruginosa Salmonella tymphimurium Shigella flexneri Bordetella pertussis Chlamydia trachomatis Yersinia spp. Pathogens of plants Erwinia amylovora Pseudomonas syringae Ralstonia solanacearum Xanthomonas campestris Pseudomonas aeruginosa Ubiquitous in addition to antibiotic-resistant bacterium Problem as long as patients with weakened immune systems Certain strains cause severe in addition to acute infections 90% of severe in addition to acute strains — inject the protein ExoU into human cells, results in cell death CDC Phospholipase A2 motifs — destroy mammalian cell membranes But, overall identity to phospholipase ~10% How does ExoU function Using genome sequences to get the answer Phillips et al. JBC (03)

Testing the phospholipase idea: Syringe Loading Clarke & McNeil. (1992) J Cell Sci 102:533-41 106 mammalian cells + ExoU ExoU kills mammalian cells in addition to needs sites 1 in addition to 2 Cell Death ExoU Site 1 Site 2 ExoU needs a mammalian cell component ExoU-GFP

Learning what that component is using yeast Yeast making ExoU Yeast without ExoU ExoU kills cells but Phospholipase inhibitors block cell death Cell Death Inhibitors Will inhibitors stop Pseudomonas Pseudomonas aeruginosa + inhibitor

Inhibitors protect against Pseudomonas Cell Death Pseudomonas w/o ExoU Pseudomonas + Inhibitor Pseudomonas Current Ann Cai Alicia Gamez Jyothi Kumaran Case McNamara Jason Miller Rebecca Phillips Catarina Raposo Loren Rodgers UCSD Ed Dennis David Six Lorraine Pillus William Fenical University of Toronto Charlie Boone Funding W.M. Keck Foundation Cystic Fibrosis Foundation

Hass, Gretchen Transworld Skateboarding Copy Editor www.phwiki.com

Hass, Gretchen Copy Editor

Hass, Gretchen is from United States and they belong to Transworld Skateboarding and they are from  Carlsbad, United States got related to this Particular Journal. and Hass, Gretchen deal with the subjects like Skating and Skateboarding; Sports

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